Merritt J, DiTonno JR, Mitra RD, Church GM, and Edwards JS. Mitra,RD, Shendure,J, Olejnik,J, Olejnik,EK, and Church,GM (2003)įluorescent in situ Sequencing on Polymerase Colonies. Single Molecule Profiling of Alternative Pre-mRNA Splicing. Zhu,J, Shendure,J, Mitra, RD, and Church, GM (2003) (2004)ĭesign of a high-throughput assay for alternative splicing using polymerase colonies.ħ. Buhler, J., Souvenir, R., Zhang, W, and Mitra, R. Aach, J and Church, GM (2004) Mathematical models of diffusion-constrained polymerase chain reactions: basis of high-throughput nucleic acid assays and simple self-organizing systems. Constans, A (2003) Beyond Sanger: Toward the $1000 genome. Pancreatic cancer cell lines by immobilized polymerase chain reaction.ġ0. 2003 Jul 22 100(15):8817-22.Ĭharacterization of mutations and loss of heterozygosity of p53 and K-ras2 in Transforming single DNA molecules into fluorescent magnetic particles for detection and enumeration of genetic variations. Dressman D, Yan H, Traverso G, Kinzler KW, and Vogelstein B. (2004) Digital Quantitative Measurements Of Gene Expression. Mikkilineni, V, Mitra, RD, Merritt, J, DiTonno, JR, Church, GM, Ogunnaike, B, and Edwards, JS. Shendure J, Mitra R, Varma C, Church GM (2004) Advanced Sequencing Technologies: Methods and Goals. Shendure, J, Porreca, GJ, Reppas, NB, Lin, X, McCutcheon, JP, Rosenbaum, AM, Wang, MD, Zhang, K, Mitra, RD, Church, GM (2005)Īccurate Multiplex Polony Sequencing of an Evolved Bacterial Genome (Science 4-Aug) Science Supplement 14. Polonies can be generated using several techniques that include solid-phase PCR in polyacrylamide gels, bridge PCR, rolling-circle amplification, BEAMing (beads, emulsions, amplification and magnetics)-based cloning on beads and massively parallel signature sequencing (MPSS) to generate clonal bead arrays."ġ5. This approach greatly improves the signal-to-noise ratio. "Polonies are discrete clonal amplifications of a single DNA molecule, grown on a solid-phase surface. Zhang, K, Martiny, AC, Reppas, NB, Barry, KW, Malek, J, Chisholm, SW, Church, GM (2006) Sequencing genomes from single cells via polymerase clones. (2007) Polony multiplex analysis of gene expression (PMAGE) in a mouse model of hypertrophic cardiomyopathy. Kim JB, Porreca GJ, Gorham JM, Church GM, Seidman CE, Seidman JG Conrad C, Jun Zhu J, Conrad C, Schoenfeld D, Fang Z, Ingelsson M, Stamm S, Church G, Hyman BT (2007) Single Molecule Profiling of tau Gene Expression in Alzheimer's Disease. Towards personalized therapy for CML patients.Ģ0. Nardi V, Raz T, Chao X, Wu CJ, Stone RM, Cortes J, Deininger MWN, Church G, Zhu J and Daley GQ (2008) Monitoring resistance to kinase inhibitors with polony technology: Church GM, Porreca GJ, Terry RC, Lares M (2008) High-speed imaging for DNA sequencing. Forest CR, Rosenbaum AM, Church GM (2008) DNA Sequencing By Ligation On Surface-Bound Beads In A Microchannel Environment. Drmanac S, Oliphant AR, Banyai WC, Martin B, Ballinger DG, Church GM, Reid CA (2009) Human Genome Sequencing Using Unchained Base Reads on Self-assembling DNA Nanoarrays. Lee J, Daugharthy ER, Scheiman J, Kalhor R, Ferrente TC, Terry R, Turczyk B, Yang JL, Lee HS, Aach J, Zhang K, Church GM (2015) Fluorescent in situ sequencing (FISSEQ) of RNA for gene expression profiling in intact cells and tissues. Open Source Polonator & In Situ Sequencing References 25. 454 vs ABI-3730xl Disclosure: Church lab licenses & advisory roles. Nucleic Acid Cloning and Sequencing by Synthesis. System-ware: Dover-Danaher documentation 2009īrought to you by: Advanced Sequencing Technology NHGRI grants.ELS-ware: Ethical, Legal, Social innovation in shareable medical research & frank discussion of data leaks and re-identification issues:.Shareware: Public discussion forum Polony-Protocols.Protocols, Software, Flow cell drawings etc. Hardware: 2009: Danaher Polonator G.007 ( 2003 & 2006 : Slide amplifiers, fuidics, scanners or bead microscope 2005).( Image analysis for in-situ haplotyping, exon-typing 2003, Polony-bead software 2005, 2006). What flavors? (1) in situ slide PCR, (2) emulsion-PCR-beads, (3) Isothermal amplification (no-PCR, no-gels, no-beads). Multiplex Cyclic Reprobing Sequencing (SbL, SbH, SbP) What is it? : Polony = Polymerase-col ony (pronounced "Pahl'ahnee" at Hahvahd, and "Pole own' ee" at the Deli). Open Source Next Generation Sequencing Technology I.
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. ArchivesCategories |